A New Species of Stream-Breeding Isthmohyla (Anura: Hylidae) from Costa Rica
Wagner Chaves-Acuña, Juan Abarca, Jonathan Navarro-Picado, Esteban Hidalgo-Mora, Marilyn Ureña-Chaves, Alejandro Valverde-Castillo, Gerardo Chaves, Federico Bolaños, and Julián Faivovich
Ichthyology & Herpetology 114: 337–356
Fig. S1. Variation in Isthmohyla nacientes. (A) Male paratype (UCR 23769). The dashed arrow points to the light-colored nuptial pad of this specimen. The solid arrow points to distinct irregular, linear scars in the dorsum. (B–C) Color variation of the limbs in adult males. (B) Male paratype (ECBAnf 50-04-0202) with orange colored limbs. (C) Adult male with yellow colored limbs (not collected).
Fig. S2. Spectrograms (above) and oscillograms (below) of vocalizations of Isthmohyla tica. (A, B, E, and F) Spectrograms. (C, D, G, and H) Oscillograms. Scale bar = 200 ms (horizontal), 1,000 Hz (vertical). (A–D) Vocalization of an adult male (KU 103754) recorded at Cinchona, central Costa Rica (audio recording ML 194065). (E–H) Vocalization of an adult male (SMF 89845) recorded at Parque Internacional La Amistad, western Panama. Audio recording shared by A. Hertz.
Fig. S3. Isthmohyla nacientes, tadpole (UCR 24429), stage 28. (A) Sloping snout. (B) Enlarged oral disc. (C) M-shaped upper jaw sheath.
Fig. S4. Isthmohyla nacientes, tadpoles in different stages. (A–C) Stage 28, UCR 23776. (D–F) Stage 35 (not collected). (G–I) Stage 41 (not collected).
Fig. S5. (A–C) Encounters of two males of Isthmohyla nacientes using the same plant. On each of these occasions, only one of the individuals was calling. White arrows pointing at individuals in the background.
Fig. S6. Strict consensus of the 66 most parsimonious trees of the analysis with gaps treated as fifth state. Values around the nodes are jackknife absolute frequencies. Nodes lacking values have frequencies <50%. An asterisk (*) indicates 100% frequency.
Fig. S7. Maximum likelihood tree obtained with IQ-TREE (pers. 0.25, ML -90934.452). The models for each partition are listed at the bottom of the figure. Values around nodes are ultrafast bootstrap frequencies. The asterisk indicates 100% frequency. Nodes with-out values have frequencies < 50%.